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. 1998 Jun;36(6):1761-4.
doi: 10.1128/JCM.36.6.1761-1764.1998.

16S rRNA sequence diversity in Mycobacterium celatum strains caused by presence of two different copies of 16S rRNA gene

U Reischl  1 , K Feldmann, L Naumann, B J Gaugler, B Ninet, B Hirschel, S Emler
Affiliations

16S rRNA sequence diversity in Mycobacterium celatum strains caused by presence of two different copies of 16S rRNA gene

U Reischl et al. J Clin Microbiol. 1998 Jun.

Abstract

Direct sequencing of the 16S rRNA gene (16S rDNA) of Mycobacterium celatum isolates showed ambiguities, suggesting heterogeneity. Cloned 16S rDNA yielded two copies of the gene, which differed by insertion of a thymine at position 214 and by additional mismatches. Restriction fragment length polymorphism analysis confirmed the presence of two copies of 16S rDNA within the bacterial chromosome.

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Figures

FIG. 1
FIG. 1
Graphic plot of the direct sequencing (lane 1) of PCR-amplified 16S rDNA and cloned 16S rDNA (clones 1 and 4) of the clinical isolate MI1581, obtained by automated sequencing. The stretch of consecutive T’s indicates the start of ambiguities in the pattern obtained by direct sequencing with reverse primer M259.
FIG. 2
FIG. 2
RFLP analysis. Southern blot of genomic DNAs of all three clinical isolates of M. celatum, i.e., 1732 (lane 1), T322 (lane 2), and MI1581 (lane 3), which were digested with PvuII and hybridized with a 16S rDNA-specific probe. The rapidly growing Mycobacterium hassiacum (lane 4) is shown as a reference. Digoxigenin-labelled DNA molecular weight marker III (Boehringer Mannheim GmbH) was applied (lane MW).
FIG. 3
FIG. 3
Alignment of a portion of 16S rDNA sequences (M. celatum types 1 (L08169) and 3 (Z46664) with clones derived from MI1581).
See this image and copyright information in PMC

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