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. 1998 Jun;36(6):1777-80.
doi: 10.1128/JCM.36.6.1777-1780.1998.

Non-O157:H7 Stx2-producing Escherichia coli strains associated with sporadic cases of hemolytic-uremic syndrome in adults

R Bonnet  1 , B Souweine, G Gauthier, C Rich, V Livrelli, J Sirot, B Joly, C Forestier
Affiliations

Non-O157:H7 Stx2-producing Escherichia coli strains associated with sporadic cases of hemolytic-uremic syndrome in adults

R Bonnet et al. J Clin Microbiol. 1998 Jun.

Abstract

From August 1996 to May 1997, six verotoxin-producing Escherichia coli (VTEC) strains were isolated from stool specimens of adults suffering from hemolytic-uremic syndrome (HUS). All the isolates were stx2 positive and belonged to different serotypes: O6:H4, O91:H10, O91:H21, O rough:H16, OX3:H-, and O nontypeable: H-. The enterohemolysin (Ehly)-encoding genes were detected in two isolates, and none of the isolates harbors the intimin (Eae)-encoding gene. These findings suggest that stx2-positive non-O157:H7 VTEC is a major cause of HUS in adults and that several sources of pathogens are responsible for local endemic infections.

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Figures

FIG. 1
FIG. 1
Agarose gel electrophoresis of DNA fragments obtained by multiplex PCR with primers specific for stx1 (140 bp) and stx2 (584 bp) (A) and with primers specific for ehly (340 bp) (B) performed with genomic extracts from different E. coli strains: EDL 933, stx1-, stx2-, and ehly-positive O157:H7 reference strain; CH011, CH013, CH014, CH015, CH016, and CH017, isolates from patients 1 through 6, respectively. M.W., 1-kb ladder of molecular size markers (Boehringer Mannheim).
See this image and copyright information in PMC

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