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. 1998 Jun;36(6):1795-7.
doi: 10.1128/JCM.36.6.1795-1797.1998.

Multiplex PCR for enterotoxigenic, attaching and effacing, and Shiga toxin-producing Escherichia coli strains from calves

S M Franck  1 , B T Bosworth, H W Moon
Affiliations

Multiplex PCR for enterotoxigenic, attaching and effacing, and Shiga toxin-producing Escherichia coli strains from calves

S M Franck et al. J Clin Microbiol. 1998 Jun.

Abstract

A multiplex PCR was developed to identify enterotoxigenic, attaching and effacing, and Shiga toxin-producing Escherichia coli strains by amplifying genes encoding K99 and F41 fimbriae, heat-stable enterotoxin a, intimin, and Shiga toxins 1 and 2. This multiplex PCR was specific and sensitive. It will be useful for identification of E. coli strains which cause diarrhea in calves.

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Figures

FIG. 1
FIG. 1
Multiplex PCR of reference strains. DNA from reference strains was used as template DNA for PCR. Products were separated on a 3% agarose gel and compared to a positive control. Bands corresponding to Stx1, eaeA, F41, K99, STa, and Stx2 are indicated. Lane A, strain B41; lane B, strain B44; lane C, strain 431; lane D, strain 637; lane E, strain 3081; lane F, strain 933; lane G, strain CHMC6; lane H, strain E2348-69; lane I, RDEC-1.
See this image and copyright information in PMC

References

    1. Braaten B A, Myers L L. Biochemical characteristics of enterotoxigenic and nonenterotoxigenic Escherichia coli isolated from calves with diarrhoea. Am J Vet Res. 1977;38:1989–1991. - PubMed
    1. Butler D G, Clarke R C. Diarrhoea and dysentery in calves. In: Gyles C L, editor. Escherichia coli in domestic animals and humans. Wallingford, United Kingdom: CAB International; 1994. pp. 91–116.
    1. Cantey J R, Inman L R. Diarrhea due to Escherichia coli strain RDEC-1 in the rabbit: the Peyer’s patch as the initial site of attachment and colonization. J Infect Dis. 1981;143:440–446. - PubMed
    1. China B, Pirson V, Mainil J. Typing of bovine attaching and effacing Escherichia coli by multiplex in vitro amplification of virulence-associated genes. Appl Environ Microbiol. 1996;62:3462–3465. - PMC - PubMed
    1. Cray W C, Jr, Moon H W. Experimental infection of calves and adult cattle with Escherichia coli O157:H7. Appl Environ Microbiol. 1995;61:1586–1590. - PMC - PubMed

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