Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1998 Jun;36(6):1801-4.
doi: 10.1128/JCM.36.6.1801-1804.1998.

A PCR specific for Escherichia coli O157 based on the rfb locus encoding O157 lipopolysaccharide

P M Desmarchelier  1 , S S Bilge, N Fegan, L Mills, J C Vary Jr, P I Tarr
Affiliations

A PCR specific for Escherichia coli O157 based on the rfb locus encoding O157 lipopolysaccharide

P M Desmarchelier et al. J Clin Microbiol. 1998 Jun.

Abstract

A PCR was developed for the detection of Escherichia coli O157 based on the rfbE O-antigen synthesis genes. A 479-bp PCR product was amplified specifically from E. coli O157 in cell lysates containing 200 or 2 CFU following crude DNA extraction. The PCR detected < 1 CFU of E. coli O157 per ml in raw milk following enrichment.

PubMed Disclaimer

Figures

FIG. 1
FIG. 1
Agarose gel of O157 rfb PCR products (A) and Southern hybridization of the gel with the O157 rfb PCR product probe (B). Lanes: 1 and 2, E. coli O157 isolates; 3 to 5, untyped animal STEC isolates; 6, O157:H7; 7, DIG λ ladder II (Boehringer Mannheim).
See this image and copyright information in PMC

References

    1. Armstrong G L, Hollingsworth J, Morris G., Jr Emerging foodborne pathogens: Escherichia coli O157:H7 as a model of entry of a new pathogen into the food supply of the developed world. Epidemiol Rev. 1996;18:29–51. - PubMed
    1. Bennett A R, MacPhee S, Betts R P. Evaluation of methods for the isolation and detection of Escherichia coli O157 in minced beef. Lett Appl Microbiol. 1995;20:375–379. - PubMed
    1. Bennett A R, MacPhee S, Betts R P. The isolation and detection of Escherichia coli O157 by use of immunomagnetic separation and immunoassay procedures. Lett Appl Microbiol. 1996;22:237–243. - PubMed
    1. Beutin L, Geier D, Zimmermann S, Karch H. Virulence markers of Shiga-like toxin-producing Escherichia coli strains originating from healthy domestic animals of different species. J Clin Microbiol. 1995;33:631–635. - PMC - PubMed
    1. Bilge S S, Vary J C, Jr, Dowell S F, Tarr P I. Role of the Escherichia coli O157:H7 O side chain in adherence and analysis of an rfb locus. Infect Immun. 1996;64:4795–4801. - PMC - PubMed

Publication types

LinkOut - more resources