Effect of undernutrition of ewes from the time of mating on fetal ovarian development in mid gestation

Abstract

Ewes were fed either 150% (High, H) or 50% (Low, L) of their energy requirements for maintenance of liveweight during early gestation. Effects of maternal nutrition on fetal ovarian size, histological structure and steroidogenic capacity were studied at Day 47 and on ovarian size and structure at Day 62 of gestation. At Day 47 of gestation, there were significantly higher concentrations of oogonia in the ovaries of L fetuses than H fetuses (105.9 v. 76.9 germ cells mm(-2); s.e. 4.94; P < 0.001). The capacity of the ovaries to secrete oestradiol (pg/ovary/24 h) at Day 47 was not affected by treatment when they were incubated either with (H, 773; L, 740; s.e. 179; not significant, n.s.) or without (H, 260; L, 290; s.e. 92.7; n.s.) ovine luteinizing hormone (oLH). At Day 62 of gestation, the process of germ cell degeneration was less advanced in L than H fetal ovaries, as indicated by higher oocyte concentrations in the former (68.4 v. 48.6 germ cells mm(-2); s.e. 3.85; P < 0 01). There was a greater percentage of meiotic cells in L ovaries (76.5 v. 18.6; s.e. 5.82; P < 0.001). It is concluded that undernutrition of the ewe from the time of mating significantly retards ovarian development in fetal ovaries.