Cross-linked polypentapeptide of tropoelastin: an insoluble, serum calcifiable matrix

Abstract

The synthetic, cross-linked polypentapeptide of tropoelastin has been shown to calcify from serum alone even when separated from the serum medium by a dialysis membrane with a low-molecular-weight cut off. By microprobe analysis, it appeared that the only serum elements required for the calcification were calcium and phosphorus. Furthermore, thin sections of the calcified matrix showed the calcification to occur throughout the matrix, and thereby verified that it is a bulk property of the matrix and not an interfacial property. To our knowledge this is the first demonstration of an insoluble, synthetic polypeptide to function as a serum calcifiable matrix and by doing so it opens the door to potential medical applications.