Reconstitution of the B800 bacteriochlorophylls in the peripheral light harvesting complex B800-850 of rhodobacter sphaeroides 2.4.1 with BChl a and modified (bacterio-)chlorophylls
- PMID: 9630729
- DOI: 10.1016/s0005-2728(98)00086-3
Reconstitution of the B800 bacteriochlorophylls in the peripheral light harvesting complex B800-850 of rhodobacter sphaeroides 2.4.1 with BChl a and modified (bacterio-)chlorophylls
Abstract
A method is described for reversibly removing bacteriochlorophyll from the B800-site of the B850-850 antenna complex from Rhodobacter sphaeroides. This method uses the oligosaccharidic detergent Triton BG-10, together with an incubation at pH 5.0. Reconstitution at the B800-site has been successfully achieved for a range of modified bacteriochlorophylls. Copyright 1998 Elsevier Science B.V. All rights reserved.
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