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Comparative Study
. 1976 Aug;57(4):589-97.
doi: 10.1111/j.1476-5381.1976.tb10389.x.

A rapid method for the estimation of prostaglandin 15-hydroxydehydrogenase activity and its application to pharmacology

G J Blackwell, R J Flower
Comparative Study

A rapid method for the estimation of prostaglandin 15-hydroxydehydrogenase activity and its application to pharmacology

G J Blackwell et al. Br J Pharmacol. 1976 Aug.

Abstract

A novel double-isotope assay for prostaglandin 15-hydroxydehydrogenase (PGDH) is described. 2 The assay is simple, rapid, precise, completely specific for PGDH, and free from artefacts. The results obtained correlate well with a conventional assay. 3 The method is versatile, being applicable to estimations of enzyme activity in cell-free systems as well as perfused organs, and is well suited to the assay of PGDH inhibitors.

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References

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