The role of taurine in osmotic, mechanical, and chemical protection of the retinal rod outer segments

Abstract

The ability of the photoreceptor cell to resist osmotic stress was examined by incubating isolated frog retina in medium of varying osmolality. An electron microscopic analysis of the rod outer segment following a severe hypoosmotic insult revealed connections between adjacent disks and between disk rims and the plasma membrane, which presumably provide mechanical stability to the rod outer segment. One surprising result was the extent of the damage incurred by the electrical signaling pathway of the photoreceptor cells subjected to a 50 mOsm insult; only the distal P111 component of the ERG remained unaffected. Thus, the rod outer segment is particularly resistant to osmotic-induced injury, presumably because of the effective osmoregulatory actions of taurine. Incubation of retina with tauret, retinylidentaurine, uncovered rose-like hexagonal structures on the surface of the rod outer segment. These structures purportedly consist of connections between disk rims and the plasma membrane of the rod outer segments. Based on the influence of tauret, it is likely that the calcium dependence of these channels is selective for retinoids. These data are discussed relative to taurine's role in the process of rhodopsin regeneration and in the protection of the rod outer segments against osmotic, mechanical and light induced damage.