doi: 10.1016/s0021-9673(97)00504-9.
Separation of pd(GC)12 from pd(AT)12 by free solution capillary electrophoresis
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- PMID: 9639887
- DOI: 10.1016/s0021-9673(97)00504-9
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Separation of pd(GC)12 from pd(AT)12 by free solution capillary electrophoresis
J Chromatogr A.
.
Abstract
The two synthetic self complementary oligonucleotides pd(AT)12 and pd(GC)12 were separated by free solution capillary electrophoresis (CZE) using simple borate buffers. The effects of pH (7.5-9) and the concentration of the buffer (0.03-0.35 M) were investigated. Higher pH values and buffer concentrations led to better resolution and longer migration times, the pH having a more pronounced effect on the separation than the concentration of the buffer. It is proposed that the conformation and effective length of the oligonucleotides may have a role in their separation in free solution capillary electrophoresis.
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