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. 1998 Jul 1;161(1):7-10.

Ly-49D and Ly-49H associate with mouse DAP12 and form activating receptors

K M Smith  1 , J Wu, A B Bakker, J H Phillips, L L Lanier
Affiliations
  • PMID: 9647200

Ly-49D and Ly-49H associate with mouse DAP12 and form activating receptors

K M Smith et al. J Immunol. .

Abstract

Several members of the Ly-49 receptor family inhibit NK cell-mediated lysis of targets expressing appropriate MHC class I molecules. Ly-49D and Ly-49H, two Ly-49 molecules that lack immunoreceptor tyrosine-based inhibitory motifs (ITIM) in their cytoplasmic domains, associate with mouse DAP12, a molecule that possesses an immunoreceptor tyrosine-based activation motif (ITAM). Cotransfection of either Ly-49D or Ly-49H with DAP12 induces surface expression of both Ly-49 and DAP12. The Ly-49/DAP12 complex was coimmunoprecipitated from the transfected cells, demonstrating a physical association of DAP12 with Ly-49D or Ly-49H in the plasma membrane. Stimulation of transfectants with Abs recognizing either Ly-49D or Ly-49H results in cellular activation, as assessed by induction of tyrosine phosphorylation of multiple cellular substrates.

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