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. 1998 Jul 15;26(14):3451-2.
doi: 10.1093/nar/26.14.3451.

Solid-phase cDNA library construction, a versatile approach

T Roeder  1
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Solid-phase cDNA library construction, a versatile approach

T Roeder. Nucleic Acids Res. .

Abstract

A rapid and versatile method for cDNA library construction was developed. It is based on conventional cDNA library synthesis including all enzymatic steps usually required, but is performed on a solid support. The cDNA is immobilised via a biotin residue to streptavidin coupled magnetic beads, which allows rapid and easy to perform changes of buffers and enzymes. Therefore, it combines speed (library construction within a single day) with high quality libraries, making it ideally suited for most purposes.

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References

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