Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1998 Jul;36(7):1948-52.
doi: 10.1128/JCM.36.7.1948-1952.1998.

Evaluation of the VITEK 2 system for rapid identification of medically relevant gram-negative rods

G Funke  1 , D Monnet, C deBernardis, A von Graevenitz, J Freney
Affiliations

Evaluation of the VITEK 2 system for rapid identification of medically relevant gram-negative rods

G Funke et al. J Clin Microbiol. 1998 Jul.

Abstract

The new VITEK 2 system (bioMérieux) was evaluated at two independent sites with the identification card for gram-negative bacilli (ID-GNB card). Of the 845 strains tested, which represented 70 different taxa belonging to either the family Enterobacteriaceae or the nonenteric bacilli, 716 (84.7%) were correctly identified at the species level. Thirty-two (3.8%) additional strains were identified to the species level after the performance of simple, rapid manual tests (oxidase, hemolysis, indole reaction, motility, and pigmentation). For 80 (9.5%) strains, these additional tests did not lead to an identification at the species level but the correct species identification was given among the organisms listed. Only 7 (0.8%) strains were misidentified, and 10 (1.2%) were not identified. Mistakes were randomly distributed over different taxa. Due to the new, more sensitive fluorescence-based technology of the VITEK 2 system, final results were available after 3 h. Since our evaluation was mainly a stress test, it is predicted that the VITEK 2 system in conjunction with the ID-GNB card would perform well under conditions of a routine clinical laboratory in identifying members of the family Enterobacteriaceae and selected species of nonenteric bacteria. This system is a promising, highly automated new tool for the rapid identification of gram-negative bacilli from human clinical specimens.

PubMed Disclaimer

References

    1. Cuziat, R., S. Cagnès, R. Cogne, M. Desmonceaux, M. Guicherd, and D. Monget. 1997. Influence of isolation media on the identification of gram-negative rods using the Vitek II system, abstr. P258. Clin. Microbiol. Infect. 3(Suppl. 2):54.
    1. Ewing W H. Edwards and Ewing’s identification of Enterobacteriaceae. 4th ed. New York, N.Y: Elsevier Science Publishing, Inc.; 1986.
    1. Farmer J J, III, Davis B R, Hickman-Brenner F W, McWorther A, Huntley-Carter G P, Asbury M A, Riddle C, Wathen-Grady H G, Elias C, Fanning G R, Steigerwalt A G, O’Hara C M, Morris G K, Smith P B, Brenner D J. Biochemical identification of new species and biogroups of Enterobacteriaceae isolated from clinical specimens. J Clin Microbiol. 1985;21:46–76. - PMC - PubMed
    1. Gayral, J. P., R. Robinson, and D. Sandstedt. 1997. A new integrated system for microbiological testing: the Vitek II system, abstr. P254. Clin. Microbiol. Infect. 3(Suppl. 2):53.
    1. Guicherd, M., S. Cagnès, R. Cogne, R. Cuziat, and M. Desmonceaux. 1997. Robustness of gram-negative rod identification with the Vitek II system, abstr. P256. Clin. Microbiol. Infect. 3(Suppl. 2):53.

Publication types

MeSH terms

Substances

LinkOut - more resources