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. 1998 Jul;36(7):2109-11.
doi: 10.1128/JCM.36.7.2109-2111.1998.

Replication of bovine herpesvirus type 4 in human cells in vitro

L Egyed  1
Affiliations

Replication of bovine herpesvirus type 4 in human cells in vitro

L Egyed. J Clin Microbiol. 1998 Jul.

Abstract

A reference strain (Movár 33/63) of bovine herpesvirus type 4 (BHV-4) was inoculated into 14 different human cell lines and five primary cell cultures representing various human tissues. BHV-4 replicated in two embryonic lung cell lines, MRC-5 and Wistar-38, and in a giant-cell glioblastoma cell culture. Cytopathic effect and intranuclear inclusion bodies were observed in these cells. PCR detected a 10,000-times-higher level of BHV-4 DNA. Titration of the supernatant indicated a 100-fold increase of infectious particles. Since this is the first bovine (human herpesvirus 8 and Epstein-Barr virus related) herpesvirus which replicates on human cells in vitro, the danger of possible human BHV-4 infection should not be ignored.

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Figures

FIG. 1
FIG. 1
Inclusion bodies of BHV-4 in nuclei of human embryonic lung cells (WI-38 cell line). Magnification, ca. ×1,000.
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