Use of fluorescent-antibody staining of cytocentrifuge-prepared smears in combination with cell culture for direct detection of respiratory viruses
- PMID: 9650977
- PMCID: PMC104993
- DOI: 10.1128/JCM.36.7.2112-2114.1998
Use of fluorescent-antibody staining of cytocentrifuge-prepared smears in combination with cell culture for direct detection of respiratory viruses
Abstract
Over a 3-year period, 1,003 respiratory samples were collected and examined for selected respiratory viruses with cytocentrifuged prepared smears stained with fluorescently labeled antibodies (IFA) in conjunction with cell culture. IFA results were compared with results obtained by cell culture. Viruses were isolated or detected by direct means in 401 samples. Agreement between culture and IFA was 90%.
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References
-
- Landry M L, Hsiung G D. Primary isolation of viruses. In: Specter S, Lancz G S, editors. Clinical virology manual. New York, N.Y: Elsevier Publishing; 1986. pp. 31–50.
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