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. 1998 Jul;36(7):2135-7.
doi: 10.1128/JCM.36.7.2135-2137.1998.

Isolation and characterization of sorbitol-fermenting Shiga toxin (Verocytotoxin)-producing Escherichia coli O157:H- strains in the Czech Republic

M Bielaszewska  1 , H Schmidt, M A Karmali, R Khakhria, J Janda, K Bláhová, H Karch
Affiliations

Isolation and characterization of sorbitol-fermenting Shiga toxin (Verocytotoxin)-producing Escherichia coli O157:H- strains in the Czech Republic

M Bielaszewska et al. J Clin Microbiol. 1998 Jul.

Abstract

Two sorbitol-fermenting (SF) Shiga toxin-producing Escherichia coli (STEC) O157:H- strains were isolated from patients with hemolytic-uremic syndrome in the Czech Republic in 1995. Their phenotypic and genotypic characteristics and genomic DNA fingerprints were identical or closely related to those of SF STEC O157:H- strains isolated in Germany in 1988 to 1997. This indicates that the Czech isolates belong to the SF STEC O157 clone which is widespread in Germany. It is the first finding of the clone outside Germany.

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Figures

FIG. 1
FIG. 1
Agarose gel showing RAPD PCR fingerprints of representative SF and NSF STEC O157 strains obtained with primer 1247. NSF E. coli O157:H7/H− strains 3075/96 (lane 1), 3010/96 (lane 2), EDL933 (lane 3), 6651/96 (lane 4), and 3817/96 (lane 5) are depicted. The SF E. coli O157:H− strains were Czech isolates 221/95 (lane 6) and 230/95 (lane 7) and German isolates 1529/97 (lane 8), 7713/95 (lane 9), and 1995/96 (lane 10). The molecular weight marker (M2) was DNA marker VI (Boehringer Gmbh); the molecular sizes of the fragments are (in base pairs) 2,176, 1,766, 1,230, 1,033, 653, 517, 453, 394, 298, 234, and 220. The internal standard (M1) consisted of a 1,600-bp and a 244-bp PCR product. In addition, internal standards were included in each lane.
FIG. 2
FIG. 2
Dendrogram derived from RAPD PCR data for Czech and German SF STEC O157:H− strains, NSF STEC O157:H7/H− strains, and SF Stx-negative E. coli O157:H19/H45 strains with the GelCompar software package. The characteristics of the strains are given in Table 2. The similarity scale is shown above the dendrogram (a similarity index of ≥80% indicates clonal relatedness).
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References

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