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. 1998 Jul;36(7):2164-6.
doi: 10.1128/JCM.36.7.2164-2166.1998.

Streptococcus iniae, a human and animal pathogen: specific identification by the chaperonin 60 gene identification method

S H Goh  1 , D Driedger, S Gillett, D E Low, S M Hemmingsen, M Amos, D Chan, M Lovgren, B M Willey, C Shaw, J A Smith
Affiliations

Streptococcus iniae, a human and animal pathogen: specific identification by the chaperonin 60 gene identification method

S H Goh et al. J Clin Microbiol. 1998 Jul.

Abstract

It was recently reported that Streptococcus iniae, a bacterial pathogen of aquatic animals, can cause serious disease in humans. Using the chaperonin 60 (Cpn60) gene identification method with reverse checkerboard hybridization and chemiluminescent detection, we identified correctly each of 12 S. iniae samples among 34 aerobic gram-positive isolates from animal and clinical human sources.

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Figures

FIG. 1
FIG. 1
Miniblot reverse checkerboard hybridization results for bacterial isolates described in Table 1. The horizontal slots contained immobilized Cpn60 600-bp target (T) DNA from S. anginosus ATCC 29971 (2T), S. bovis ATCC 9809 (3T), S. equi ATCC 9528 (4T), S. mitis ATCC 9811 (5T), S. mutans ATCC 25175 (6T) S. pneumoniae ATCC 27336 (7T), S. porcinus SR1387/93 (8T), S. salivarius ATCC 25957 (9T), S. pyogenes ATCC 19615 (10T), S. agalactiae ATCC 12386 (11T), S. iniae ATCC 29178 (12T), Staphylococcus aureus ATCC 12600 (13T), Citrobacter freundii ATCC 8090 (14T), Enterobacter aerogenes ATCC 13048 (15T), Escherichia coli ATCC 25922 (16T), Klebsiella pneumoniae ATCC 13883 (17T), Proteus vulgaris ATCC 13315 (18T), Salmonella typhimurium ATCC 14028 (19T), Serratia marcescens ATCC 8100 (20T), Shigella sonnei ATCC 25931 (21T), Neisseria gonorrhoeae ATCC 41426 (22T), Bacillus cereus ATCC 11778 (23T), Yersinia enterocolitica ATCC 27729 (24T), Proteus mirabilis ATCC 25933 (25T), Staphylococcus lugdunensis ATCC 43804 (26T), Borrelia burgdorferi ATCC 53899 (27T), Streptococcus faecalis ATCC 19433 (28T), and Staphylococcus epidermidis ATCC 14990 (29T). Slots 1T and 30T contained no DNA. The vertical hybridization channels contained digoxigenin-labelled Cpn60 600-bp probe (P) DNA from S. iniae ATCC 29178 (1P), isolate 582 (3P), isolate 608 (5P), isolate 286 (7P), isolate 288 (9P), isolate 9001-1 (11P), isolate 9001-2 (12P), isolate 9001-3 (13P), isolate 9001-4 (14P), isolate 9002-1 (15P), isolate 9002-2 (16P), isolate 9002-3 (17P), isolate 9002-4 (18P), isolate 9003-2 (19P), isolate 9003-3 (20P), isolate 9003-4 (21P), isolate 9003-5 (22P), isolate 9004-1 (23P), isolate 9004-2 (24P), isolate 9004-3 (25P), isolate 9005-1 (26P), isolate 9005-2 (27P), isolate 9005-3 (28P), isolate 9005-4 (29P), isolate 9005-5 (30P), isolate 9005-6 (31P), isolate 9005-7 (32P), isolate 9006-1 (33P), isolate 9006-2 (34P), isolate 9006-3 (35P), isolate 9019 (36P), isolate 9020 (37P), isolate 9085 (38P), and isolate 9086 (39P). Vertical hybridization channels 2P, 4P, 6P, 8P, 10P, and 40P contained hybridization buffer alone.
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References

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