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. 1998 Jul;40(1):58-60.
doi: 10.1006/plas.1998.1341.

Identification of linear DNA plasmids of the yeast Pichia pastoris

H Banerjee  1 , C Kopvak, D Curley
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Identification of linear DNA plasmids of the yeast Pichia pastoris

H Banerjee et al. Plasmid. 1998 Jul.

Abstract

Two DNA plasmids, approximately 11 and 8 kb in size, have been identified in a strain of the yeast Pichia pastoris (Northern Regional Research Laboratories No. Y4290). The plasmids are resistant to RNase A and lambda exonuclease, but are sensitive to digestion by DNase I, suggesting that they are linear and double-stranded DNA with 5'-protected ends. A restriction map has been constructed for the 11-kb plasmid, confirming that it is linear.

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