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. 1998 Jul;16(7):642-6.
doi: 10.1038/nbt0798-642.

Transgenic bovine chimeric offspring produced from somatic cell-derived stem-like cells

J B Cibelli  1 , S L Stice, P J Golueke, J J Kane, J Jerry, C Blackwell, F A Ponce de León, J M Robl
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Transgenic bovine chimeric offspring produced from somatic cell-derived stem-like cells

J B Cibelli et al. Nat Biotechnol. 1998 Jul.

Abstract

We have developed a method, using nuclear transplantation, to produce transgenic embryonic stem (ES)-like cells from fetal bovine fibroblasts. These cells, when reintroduced into preimplantation embryos, differentiated into derivatives from the three embryonic germ layers, ectoderm, mesoderm, and endoderm, in 5-month-old animals. Six out of seven (86%) calves born were found to be chimeric for at least one tissue. These experiments demonstrate that somatic cells can be genetically modified and then de-differentiated by nuclear transfer into ES-like cells, opening the possibility of using them in differentiation studies and human cell therapy.

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  • From cows stem therapies?
    First NL, Thomson J. First NL, et al. Nat Biotechnol. 1998 Jul;16(7):620-1. doi: 10.1038/nbt0798-620. Nat Biotechnol. 1998. PMID: 9661190 No abstract available.

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