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. 1998 Jul;5(4):499-502.
doi: 10.1128/CDLI.5.4.499-502.1998.

Concentrations of circulating beta-chemokines do not correlate with viral load in human immunodeficiency virus-infected individuals

V N Kakkanaiah  1 , E A Ojo-Amaize, J B Peter
Affiliations

Concentrations of circulating beta-chemokines do not correlate with viral load in human immunodeficiency virus-infected individuals

V N Kakkanaiah et al. Clin Diagn Lab Immunol. 1998 Jul.

Abstract

The CC or beta-chemokines MIP-1alpha, MIP-1beta, and RANTES are the primary components of human immunodeficiency virus type 1 (HIV-1)-suppressive soluble factors in vitro. We studied the relationship between the concentrations of MIP-1alpha, MIP-1beta, and RANTES in plasma and HIV viral load in HIV-infected subjects. The HIV-positive patient group (n = 140) had significantly lower concentrations of all three beta-chemokines (MIP-1alpha, P < 0.0005; MIP-1beta, P < 0.005; RANTES, P < 0.0005) than the control group (n = 58 for MIP-1alpha, n = 27 for MIP-1beta, and n = 59 for RANTES). In addition, we divided the patient group into three subgroups (high, moderate, and low) based on the number of HIV-1 RNA copies in the plasma (as measured by quantitative HIV RNA PCR). Again, all three subgroups had significantly lower concentrations of the beta-chemokines than the HIV-negative control group. However, there was no significant difference in plasma beta-chemokine concentrations among the three subgroups within the patient group (P < 0.3). Although our results demonstrate that HIV-infected individuals had significantly lower concentrations of circulating beta-chemokines than healthy uninfected control subjects, we found no correlation between the concentrations of beta-chemokines in plasma and HIV-1 viral load in HIV-infected individuals.

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Figures

FIG. 1
FIG. 1
MIP-1α concentrations in HIV+ patients. Plasma samples from healthy controls and HIV+ patients were analyzed for MIP-1α concentrations by EIA. Means are indicated (diamonds).
FIG. 2
FIG. 2
MIP-1β concentrations in HIV+ patients. The plasma samples from healthy controls and HIV+ patients were analyzed for MIP-1β concentrations by EIA. Means are indicated (diamonds).
FIG. 3
FIG. 3
RANTES concentrations in HIV+ patients. The plasma samples from healthy controls and HIV+ patients were analyzed for RANTES concentrations by EIA. Means are indicated (diamonds).
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