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. 1998;12(4):238-41.
doi: 10.1002/(sici)1098-2825(1998)12:4<238::aid-jcla8>3.0.co;2-3.

Evaluation of PCR assays in presence of antibody to thermostable DNA polymerases for detection of microbial agents: avoiding false negative results for specimen containing low-titer agent

S K Poddar  1 , M H Sawyer, J D Connor
Affiliations

Evaluation of PCR assays in presence of antibody to thermostable DNA polymerases for detection of microbial agents: avoiding false negative results for specimen containing low-titer agent

S K Poddar et al. J Clin Lab Anal. 1998.

Abstract

The serial low-titer specimens of Influenza A virus and Adeno virus type 7 were tested for the presence of virus specific genes by PCR based on Tth DNA polymerase and by that based on Taq DNA polymerase, in the absence and presence of antibody to the respective DNA polymerases. Increased product DNA synthesis and higher sensitivity of detection were observed in the presence of antibody compared to those in the absence of antibody. 10- to 100- fold lower titer specimen of Influenza A virus and 10-fold lower titer specimen of Adeno virus could be detected in the presence of antibody than those detected in the absence of antibody to the appropriate DNA polymerase, in a PCR.

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References

    1. Innis MA, Gelfand DH: Optimization of PCR In PCR Protocols: A Guide to Methods and Applications. Innis M. A., Gelfand D. H., Sninsky J. J., and White T. J., eds. Academic Press, Inc., New York, 1990.
    1. Pring‐Akerblom P, Adrian T: Type and group specific polymerase chain reaction for adenovirus detection. Res Virol 145: 25–35, 1994. - PubMed
    1. Wright KE, Wilson GAR, Novosad D, Dimock C, Tan D, Weber JM: Typing and subtyping of influenza viruses in clinical samples by PCR. J Clin Microbiol 33: 1180–1184, 1995. - PMC - PubMed
    1. Fujimura RK, Shapshak P, Feaster D, Epler M, Goodkin K: A rapid method for comparative quantitative polymerase chain reaction of HIV‐1 proviral DNA extracted from cryopreserved brain tissues. J Virol Meth ods 61: 117–187 1997. - PubMed
    1. Mulder J, McKinney N, Christopherson C, Sninnsky J, Greenfield L, Kwok S: Rapid and simple PCR assay for quantitation of human immunodeficiency virus type 1 RNA in plasma: Application to acute infection. J Clin Microbiol 32: 292–300, 1994. - PMC - PubMed

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