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. 1998 Jul 10;94(1):45-53.
doi: 10.1016/s0092-8674(00)81220-9.

A subset of TAF(II)s are integral components of the SAGA complex required for nucleosome acetylation and transcriptional stimulation

P A Grant  1 , D Schieltz, M G Pray-Grant, D J Steger, J C Reese, J R Yates 3rd, J L Workman
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Free article

A subset of TAF(II)s are integral components of the SAGA complex required for nucleosome acetylation and transcriptional stimulation

P A Grant et al. Cell. .
Free article

Abstract

A number of transcriptional coactivator proteins have been identified as histone acetyltransferase (HAT) proteins, providing a direct molecular basis for the coupling of histone acetylation and transcriptional activation. The yeast Spt-Ada-Gcn5-acetyltransferase (SAGA) complex requires the coactivator protein Gcn5 for HAT activity. Identification of protein subunits by mass spectrometry and immunoblotting revealed that the TATA binding protein-associated factors (TAF(II)s) TAF(II)90, -68/61, -60, -25/23, and -20/17 are integral components of this complex. In addition, TAF(II)68 was required for both SAGA-dependent nucleosomal HAT activity and transcriptional activation from chromatin templates in vitro. These results illustrate a role for certain TAF(II) proteins in the regulation of gene expression at the level of chromatin modification that is distinct from the TFIID complex and TAF(II)145.

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Comment in

  • The TAFs in the HAT.
    Struhl K, Moqtaderi Z. Struhl K, et al. Cell. 1998 Jul 10;94(1):1-4. doi: 10.1016/s0092-8674(00)81213-1. Cell. 1998. PMID: 9674419 Review. No abstract available.

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