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. 1976 Jul;3(7):1747-60.
doi: 10.1093/nar/3.7.1747.

Two restriction endonucleases from Bacillus globiggi

V Pirrotta
Free PMC article

Two restriction endonucleases from Bacillus globiggi

V Pirrotta. Nucleic Acids Res. 1976 Jul.
Free PMC article

Abstract

The sites of action of the restriction enzyme Bgl II on lambda DNA are mapped. This enzyme recognises the sequence 5' ...AGATCT...3' and makes staggered cuts producing sticky ends. In lambda DNA, the second A in this sequence is methylated about 50% of the time by a bacterial methylase absent in E. coli dam. In contrast to Bgl II, Bgl I makes many cuts in lambda DNA and produces 5' terminals which are not substrates for polynucleotide kinase.

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