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. 1978 Jun 1;171(3):821-4.
doi: 10.1042/bj1710821.

Phospholipid content and activity of pure uridine diphosphate-glucuronyltransferase from rat liver

B Burchell, T Hallinan

Phospholipid content and activity of pure uridine diphosphate-glucuronyltransferase from rat liver

B Burchell et al. Biochem J. .

Abstract

Rat liver phospholipids were radioactively labeled in vivo before purification of UDP-glucuronyltransferase to homogeneity. The pure enzyme contained very little phospholipid (approx. 0.7 mol of phospholipid/mol of protein). The solubilization detergent Lubrol 12A9 appeared to act as a phospholipid substitute, capable of supporting UDP-glucuronyltransferase activity. Phospholipase C did not inhibit the pure enzyme activity and pure UDP-glucuronyltransferase was stimulated by 40--100% by the addition of phospholipid dispersions.

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