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Comparative Study
. 1998 Aug 15;26(16):3865-6.
doi: 10.1093/nar/26.16.3865.

Two color hybridization analysis using high density oligonucleotide arrays and energy transfer dyes

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Comparative Study

Two color hybridization analysis using high density oligonucleotide arrays and energy transfer dyes

J G Hacia et al. Nucleic Acids Res. .

Abstract

High density oligonucleotide arrays (DNA chips) have been used in two color mutational analysis of the 3.43 kb exon 11 of the hereditary breast and ovarian cancer gene BRCA1 . Two color analysis allows competitive hybridization between a reference standard and an unknown sample, improving the performance of the assay. Fluorescein and phycoerythrin dyes werepreviously used due to their compatibility with a single line 488 nm excitation source. Here we show that an alternative dye combination, containing the energy transfer dye system phycoerythrin*cy5 along with phycoerythrin, provides more evenly matched signal intensities and decreased spectral overlap between the two fluorophores, while maintaining compatibility with a 488 nm excitation source.

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References

    1. Proc Natl Acad Sci U S A. 1995 May 9;92(10):4347-51 - PubMed
    1. Nat Genet. 1996 Dec;14(4):441-7 - PubMed
    1. Science. 1996 Oct 25;274(5287):610-4 - PubMed

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