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Comparative Study
. 1998 Aug 15;26(16):3869-70.
doi: 10.1093/nar/26.16.3869.

A rapid and sensitive assay for histone acetyl-transferase activity

S Ait-Si-Ali  1 , S Ramirez, P Robin, D Trouche, A Harel-Bellan
Affiliations
Comparative Study

A rapid and sensitive assay for histone acetyl-transferase activity

S Ait-Si-Ali et al. Nucleic Acids Res. .

Abstract

Histone acetyl-transferases (HATs) seem to be key elements in the regulation of transcription. We have designed an enzymatic assay to quantify HAT enzymatic activity. In this assay, the substrate is a peptide corresponding to the 24 first amino acids of histone H4 which is coupled to biotin. After acetylation using [14C]acetyl-CoA, the peptide is purified on streptavidin beads and the associated radioactivity is measured. This assay is sensitive, rapid and convenient.

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