Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 1998 Jul 3;430(3):419-23.
doi: 10.1016/s0014-5793(98)00706-6.

Detection and quantitation of cellularly derived amyloid beta peptides by immunoprecipitation-HPLC-MS

N J Clarke  1 , A J Tomlinson, Y Ohyagi, S Younkin, S Naylor
Affiliations
Free article
Comparative Study

Detection and quantitation of cellularly derived amyloid beta peptides by immunoprecipitation-HPLC-MS

N J Clarke et al. FEBS Lett. .
Free article

Abstract

A quantitative method for detection of amyloid beta peptides using immunoprecipitation-HPLC-mass spectrometry (IP-LC-MS) is described. Comparison of IP-LC-MS with sandwich ELISA revealed comparable results in the analysis of A beta 1-40 and A beta 1-42 derived from fetal guinea pig cell media and cell lysates. The use of IP-LC-MS not only allows a quantitative method for A beta 1-40 and A beta 1-42 peptides present in Alzheimer's disease (AD), but allows detection of other A beta peptide species that may also play a role in the onset of AD in humans.

PubMed Disclaimer

Publication types

LinkOut - more resources