Immunolocalization of sat-1 sulfate/oxalate/bicarbonate anion exchanger in the rat kidney
- PMID: 9689008
- DOI: 10.1152/ajprenal.1998.275.1.F79
Immunolocalization of sat-1 sulfate/oxalate/bicarbonate anion exchanger in the rat kidney
Abstract
The rat liver sulfate/bicarbonate/oxalate exchanger (sat-1) transports sulfate across the canalicular membrane in exchange for either bicarbonate or oxalate. Sulfate/oxalate exchange has been detected in the proximal tubule of the kidney, where it is probably involved in the reabsorption of filtered sulfate and the secretion of oxalate and may contribute to oxalate-dependent chloride reabsorption. Screening of a renal cortex cDNA library determined that sat-1 is expressed in the rat kidney. To evaluate this anion exchanger, the sat-1 protein was expressed in Sf9 cells. Sodium-independent sulfate and oxalate uptake was enhanced 7.3-fold and 13.1-fold, respectively, in Sf9 cells expressing the sat-1 protein compared with cells infected with wild-type virus. We determined that sat-1 is glycosylated in the kidney; however, anion exchange via sat-1 is observed despite incomplete glycosylation of sat-1 in Sf9 cells. The sat-1 protein, with an added COOH-terminal 6-histidine tag, was purified on a metal affinity column and used to generate anti-sat-1 monoclonal antibodies. The sat-1 protein was localized to the basolateral membrane, but not the apical membrane, of the proximal tubule by both Western blot analysis and immunohistochemistry. These studies demonstrate that sulfate/oxalate exchange on the apical and basolateral membranes of the proximal tubule represents transport on two different anion exchangers.
Similar articles
-
The liver and kidney expression of sulfate anion transporter sat-1 in rats exhibits male-dominant gender differences.Pflugers Arch. 2009 Apr;457(6):1381-92. doi: 10.1007/s00424-008-0611-5. Epub 2008 Nov 11. Pflugers Arch. 2009. PMID: 19002488
-
Effects of sulfate and chloride on three separate oxalate transporters reconstituted from rabbit renal cortex.Am J Physiol. 1998 Jan;274(1):F189-96. doi: 10.1152/ajprenal.1998.274.1.F189. Am J Physiol. 1998. PMID: 9458839
-
Oxalate transport via the sulfate/HCO3 exchanger in rabbit renal basolateral membrane vesicles.J Biol Chem. 1988 Jul 15;263(20):9710-7. J Biol Chem. 1988. PMID: 3384817
-
Ion exchangers mediating NaCl transport in the proximal tubule.Wien Klin Wochenschr. 1997 Jun 27;109(12-13):435-40. Wien Klin Wochenschr. 1997. PMID: 9261983 Review.
-
Essential roles of CFEX-mediated Cl(-)-oxalate exchange in proximal tubule NaCl transport and prevention of urolithiasis.Kidney Int. 2006 Oct;70(7):1207-13. doi: 10.1038/sj.ki.5001741. Epub 2006 Aug 2. Kidney Int. 2006. PMID: 16883319 Review.
Cited by
-
Hyperoxaluric rats do not exhibit alterations in renal expression patterns of Slc26a1 (SAT1) mRNA or protein.Urol Res. 2012 Dec;40(6):647-54. doi: 10.1007/s00240-012-0480-4. Epub 2012 May 10. Urol Res. 2012. PMID: 22573180 Free PMC article.
-
Functional analysis of nonsynonymous single nucleotide polymorphisms in human SLC26A9.Hum Mutat. 2012 Aug;33(8):1275-84. doi: 10.1002/humu.22107. Epub 2012 Jun 7. Hum Mutat. 2012. PMID: 22544634 Free PMC article.
-
Acute regulation of the SLC26A3 congenital chloride diarrhoea anion exchanger (DRA) expressed in Xenopus oocytes.J Physiol. 2003 May 15;549(Pt 1):3-19. doi: 10.1113/jphysiol.2003.039818. Epub 2003 Mar 21. J Physiol. 2003. PMID: 12651923 Free PMC article.
-
Progress in Understanding the Genetics of Calcium-Containing Nephrolithiasis.J Am Soc Nephrol. 2017 Mar;28(3):748-759. doi: 10.1681/ASN.2016050576. Epub 2016 Dec 8. J Am Soc Nephrol. 2017. PMID: 27932479 Free PMC article. Review.
-
Pathogens that infect mammalian cells via sulfonated glycosaminoglycans.Front Cell Infect Microbiol. 2025 Jun 10;15:1613923. doi: 10.3389/fcimb.2025.1613923. eCollection 2025. Front Cell Infect Microbiol. 2025. PMID: 40557318 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Research Materials