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. 1998 Aug;180(16):4300-2.
doi: 10.1128/JB.180.16.4300-4302.1998.

Agrobacterium VirE2 proteins can form a complex with T strands in the plant cytoplasm

S B Gelvin  1
Affiliations

Agrobacterium VirE2 proteins can form a complex with T strands in the plant cytoplasm

S B Gelvin. J Bacteriol. 1998 Aug.

Abstract

Wild-type VirE2 and VirD2 proteins from Agrobacterium tumefaciens contain nuclear targeting sequences (NLS) that are likely involved in directing transferred T strands to the plant nucleus. An A. tumefaciens virE2 virD2DeltaNLS double mutant was able to form tumors on VirE2-producing transgenic tobacco but not on wild-type tobacco. Because this mutant bacterial strain contains no known T-strand nuclear targeting signal, the data indicate that wild-type VirE2 proteins produced by the plant can interact with the T strands in the plant cytoplasm and direct them to the nucleus.

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References

    1. Binns A N, Beaupre C E, Dale E M. Inhibition of VirB-mediated transfer of diverse substrates from Agrobacterium tumefaciens by the IncQ plasmid RSF1010. J Bacteriol. 1995;177:4890–4899. - PMC - PubMed
    1. Christie P J, Ward J E, Winans S C, Nester E W. The Agrobacterium tumefaciens virE2 gene product is a single-stranded DNA-binding protein that associates with T-DNA. J Bacteriol. 1988;170:2659–2667. - PMC - PubMed
    1. Citovsky V, De Vos G, Zambryski P. Single-stranded DNA binding protein encoded by the virE locus of Agrobacterium tumefaciens. Science. 1988;240:501–504. - PubMed
    1. Citovsky V, Warnick D, Zambryski P. Nuclear import of Agrobacterium VirD2 and VirE2 proteins in maize and tobacco. Proc Natl Acad Sci USA. 1994;91:3210–3214. - PMC - PubMed
    1. Citovsky V, Wong M L, Zambryski P. Cooperative interaction of Agrobacterium VirE2 protein with single-stranded DNA: implications for the T-DNA transfer process. Proc Natl Acad Sci USA. 1989;86:1193–1197. - PMC - PubMed

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