Agrobacterium VirE2 proteins can form a complex with T strands in the plant cytoplasm
- PMID: 9696783
- PMCID: PMC107431
- DOI: 10.1128/JB.180.16.4300-4302.1998
Agrobacterium VirE2 proteins can form a complex with T strands in the plant cytoplasm
Abstract
Wild-type VirE2 and VirD2 proteins from Agrobacterium tumefaciens contain nuclear targeting sequences (NLS) that are likely involved in directing transferred T strands to the plant nucleus. An A. tumefaciens virE2 virD2DeltaNLS double mutant was able to form tumors on VirE2-producing transgenic tobacco but not on wild-type tobacco. Because this mutant bacterial strain contains no known T-strand nuclear targeting signal, the data indicate that wild-type VirE2 proteins produced by the plant can interact with the T strands in the plant cytoplasm and direct them to the nucleus.
References
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- Citovsky V, De Vos G, Zambryski P. Single-stranded DNA binding protein encoded by the virE locus of Agrobacterium tumefaciens. Science. 1988;240:501–504. - PubMed
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