doi: 10.1038/nbt0898-737.
Identification of expressed genes by laser-mediated manipulation of single cells
Affiliations
- PMID: 9702771
- DOI: 10.1038/nbt0898-737
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Identification of expressed genes by laser-mediated manipulation of single cells
Nat Biotechnol.
1998 Aug.
Abstract
We describe a rapid noncontact method for the capture of single cells or small tissue areas of any size or shape directly within the cap of a common microfuge tube. Prior to the laser-mediated transfer, the specimen is isolated by laser microbeam microdissection, forming a clear-cut gap around the selected area. Laser treatment does not impair subsequent RNA analysis. We have used this method to isolate a single cell from archival colon adenocarcinoma, and were able to detect point mutations within codon 12 of c-Ki-ras2 mRNA after nested RT-PCR analysis.
Comment in
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Select, microdissect, and eject.Nat Biotechnol. 1998 Aug;16(8):719-20. doi: 10.1038/nbt0898-719. Nat Biotechnol. 1998. PMID: 9702764 No abstract available.
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