doi: 10.1007/BF01971570.
Rapid purification of human high molecular weight kininogen
- PMID: 970286
- DOI: 10.1007/BF01971570
Item in Clipboard
Rapid purification of human high molecular weight kininogen
Agents Actions.
1976 Sep.
Abstract
Human high molecular weight kininogen was isolated by a rapid procedure, using anion exchange chromatography on QAE-Sephadex, ammonium sulfate precipitation and cation exchange chromatography on CM-Sephadex. The poor recovery and relatively low specific activity observed in earlier experiments was found to be due to a contaminant, presumably enzymatic, capable of releasing kinin from the kininogen. The "spontaneous" kinin release was blocked by soy bean trypsin inhibitor and by C1-inactivator. The isolated kininogen was stable at different temperatures, did not contain free kinin and was a good substrate for plasma kallikrein and plasmin.
Similar articles
-
Purification of human low molecular weight kininogen and its application for a simple ND sensitive method for determination of human urinary kallikrein activity.Adv Exp Med Biol. 1986;198 Pt B:523-9. doi: 10.1007/978-1-4757-0154-8_66. Adv Exp Med Biol. 1986. PMID: 3643739
-
Purification and characterization of rat low molecular weight kininogen.J Biochem. 1984 Jul;96(1):81-8. doi: 10.1093/oxfordjournals.jbchem.a134832. J Biochem. 1984. PMID: 6238024
-
Purification of a high molecular weight kininogen from rat plasma.Prep Biochem. 1980;10(5):561-79. doi: 10.1080/00327488008061754. Prep Biochem. 1980. PMID: 7443632
-
Studies on human kininogens. I. Isolation, characterization, and cleavage by plasma kallikrein of high molecular weight (HMW)-kininogen.J Biochem. 1979 Jan;85(1):249-58. doi: 10.1093/oxfordjournals.jbchem.a132318. J Biochem. 1979. PMID: 104988
-
Rodent kinin-forming enzyme systems--I. Purification and characterization of plasma kininogen.Biochem Pharmacol. 1983 Jul 1;32(13):2061-9. doi: 10.1016/0006-2952(83)90427-6. Biochem Pharmacol. 1983. PMID: 6870934
Cited by
-
High-molecular weight kininogen. A secreted platelet protein.J Clin Invest. 1983 May;71(5):1477-89. doi: 10.1172/jci110901. J Clin Invest. 1983. PMID: 6406551 Free PMC article.
-
Surface activation of factor XII (Hageman factor)--critical role of high molecular weight kininogen and another potentiator).Agents Actions. 1978 Jan;8(1-2):65-72. doi: 10.1007/BF01972404. Agents Actions. 1978. PMID: 636957
-
Function and immunochemistry of prekallikrein-high molecular weight kininogen complex in plasma.J Clin Invest. 1980 Feb;65(2):413-21. doi: 10.1172/JCI109684. J Clin Invest. 1980. PMID: 7356688 Free PMC article.