Mapping of several abnormal hemoglobins by horizontal polyacrylamide gel isoelectric focusing
- PMID: 970376
- DOI: 10.1093/ajcp/66.4.753
Mapping of several abnormal hemoglobins by horizontal polyacrylamide gel isoelectric focusing
Abstract
The relative mobilities of serveral human hemoglobin variants were studied by use of an isoelectric focusing method in a thin-layer horizontal polyacrylamide gel. The technic is described in detail and a preliminary mapping of these variants is presented. It appears that thin-layer gel isoelectric focusing is a method that is rapid, highly reproducible and easily applicable in laboratories concerned with the study of hemoglobinopathies.
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