Microfilarial periodicity of Wuchereria bancrofti and man landing periodicity of the vector Culex quinquefasciatus say in Matara, Sri Lanka

Abstract

Objective: To compare the microfilarial periodicity of Wuchereria bancrofti, with the man landing periodicity of the vector Culex quinquefasciatus in Matara, Sri Lanka.

Design: Periodicity was estimated using a statistical method. 60 microliters finger prick (FP) blood was smeared from a single subject every 2 hours for 24 hours of the day to make 12 samples. Smears were stained with Giemsa and the microfilariae (mff) counted. Man landing catches of mosquitoes were made inside a bedroom of a house in the same area on a sleeping volunteer during the night, between 18.00 and 06.00 hours. Each hourly catch was placed in separate paper cups. Hourly C. quinquefasciatus taken were counted.

Subjects: 10 asymptomatic microfilaria (mf) carriers.

Results: The individual mf peaks in the 10 carriers varied from 22.00 to 04.00 hours. Using the statistical method the parameter k showing the mf peak hour was 1.19 estimating the peak mf density at 01.11 hours. The influence of different times of blood collection on false negatives among the very low density carriers was estimated by the periodicity curve. It would be desirable to collect blood during the estimated time interval when the mf count was 80% of the peak count, between 21.55 and 04.27 hours in Matara. The results of 25 all-night mosquito landing catches gave a peak activity hours of k as 7.78, corresponding to 01.47 hours.

Conclusion: The close agreement in the peak hours of mf density and vector activity suggests a perfect adaptation between parasite and vector for optimum transmission.