Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1998 Sep;36(9):2766-8.
doi: 10.1128/JCM.36.9.2766-2768.1998.

Clinical evaluation of the BDProbeTec strand displacement amplification assay for rapid diagnosis of tuberculosis

J S Bergmann  1 , G L Woods
Affiliations

Clinical evaluation of the BDProbeTec strand displacement amplification assay for rapid diagnosis of tuberculosis

J S Bergmann et al. J Clin Microbiol. 1998 Sep.

Abstract

The reliability of the BDProbeTec MTB Test (Becton Dickinson, Sparks, Md.) for direct detection of Mycobacterium tuberculosis in respiratory specimens was evaluated by comparing results to those of conventional mycobacterial culture, with the BACTEC TB 460 and Middlebrook 7H11 biplates. Patients known to have tuberculosis were excluded from analysis. Of 523 specimens from 277 patients, 53 grew a mycobacterium: 24 specimens of M. tuberculosis and 29 specimens of nontuberculous mycobacteria. After initial testing, 42 specimens were positive by the BDProbeTec, for overall sensitivity, specificity, and positive and negative predictive values of 95.8, 96. 2, 54.8, and 99.8%, respectively. After resolution of discrepancies, 28 specimens were positive by the BDProbeTec, for overall sensitivity, specificity, and positive and negative predictive values of 100, 99.2, 85.7, and 100%, respectively. These same values were 100, 80.8, 93.4, and 100%, respectively, for smear-positive samples and 100, 99.4, 75.0, and 100%, respectively, for smear-negative specimens.

PubMed Disclaimer

References

    1. American Thoracic Society Workshop. Rapid diagnostic tests for tuberculosis. What is the appropriate use? Am J Respir Crit Care Med. 1997;155:1804–1814. - PubMed
    1. Bergmann J S, Woods G L. Clinical evaluation of the Roche Amplicor PCR Mycobacterium tuberculosis test for detection of M. tuberculosis in respiratory specimens. J Clin Microbiol. 1996;34:1083–1085. - PMC - PubMed
    1. Dalovisio J R, Montenegro-James S, Kemmerly S A, Genre C F, Chambers R, Greer D, Pankey G A, Failla D M, Haydel K G, Hutchinson L, Lindley M F, Nunez B M, Praba A, Eisenach K D, Cooper E S. Comparison of the Amplified Mycobacterium tuberculosis (MTB) Direct Test, Amplicor MTB PCR, and IS6110-PCR for detection of MTB in respiratory specimens. Clin Infect Dis. 1996;23:1099–1106. - PubMed
    1. Gamboa F, Manterola J M, Vinado B, Matas L, Gimenez M, Lonca J, Manzano J R, Rodrigo C, Cardona P J, Padilla E, Dominguez J, Ausina V. Direct detection of Mycobacterium tuberculosis complex in nonrespiratory specimens by Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test. J Clin Microbiol. 1997;35:307–310. - PMC - PubMed
    1. Ichiyama S, Iinuma Y, Tawada Y, Yamora S, Hasegawa Y, Shimokata K, Nakashima N. Evaluation of Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test and Roche PCR-microwell plate hybridization method (Amplicor Mycobacterium) for direct detection of mycobacteria. J Clin Microbiol. 1996;34:130–133. - PMC - PubMed

Publication types

MeSH terms

LinkOut - more resources