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. 1998 Sep;36(9):2778-81.
doi: 10.1128/JCM.36.9.2778-2781.1998.

Development of specific nested oligonucleotide PCR primers for the Streptococcus iniae 16S-23S ribosomal DNA intergenic spacer

B R Berridge  1 , J D Fuller, J de Azavedo, D E Low, H Bercovier, P F Frelier
Affiliations

Development of specific nested oligonucleotide PCR primers for the Streptococcus iniae 16S-23S ribosomal DNA intergenic spacer

B R Berridge et al. J Clin Microbiol. 1998 Sep.

Abstract

Streptococcus iniae is a cause of septicemia, meningoencephalitis, and death in farmed fish and of cellulitis in human beings. A set of nested oligonucleotide PCR primers that specifically amplified a 373-bp subunit from a variety of clinical isolates from farmed fish and human patients were constructed from a 524-bp consensus sequence of the S. iniae 16S-23S ribosomal DNA intergenic spacer.

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Figures

FIG. 1
FIG. 1
S. iniae-specific PCR primer design scheme. The 16S-23S rDNA intergenic spacer was nonspecifically PCR amplified from the S. iniae rRNA operon. S. iniae-specific oligonucleotide primers 5′144 and 3′516 define a 373-bp subunit.
See this image and copyright information in PMC

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