Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 1998 Sep;66(9):4011-7.
doi: 10.1128/IAI.66.9.4011-4017.1998.

Counterselectable markers: untapped tools for bacterial genetics and pathogenesis

Affiliations
Review

Counterselectable markers: untapped tools for bacterial genetics and pathogenesis

J M Reyrat et al. Infect Immun. 1998 Sep.
No abstract available

PubMed Disclaimer

Figures

FIG. 1
FIG. 1
(A) Double-selection strategy. Positive selection of allelic exchange mutants in a two-step selection strategy, using a counterselectable marker. (B) Single-selection strategy. CSM, counterselectable marker; SM, selectable marker; WT, wild-type allele; mut, mutated allele.

Similar articles

Cited by

References

    1. Azad A K, Sirakova T D, Fernandes N D, Kolattukudy P E. Gene knockout reveals a novel gene cluster for the synthesis of a class of cell wall lipids unique to pathogenic mycobacteria. J Biol Chem. 1997;272:16741–16745. - PubMed
    1. Berg C M, Berg D E. Transposable element tools for microbial genetics. In: Neidhardt F C, Curtis III R, Ingraham J L, Lin E C C, Low K B, Magasanik B, Reznikoff W S, Riley M, Schaechter M, Umbarger H E, editors. Escherichia coli and Salmonella: cellular and molecular biology. Washington, D.C: ASM Press; 1996. pp. 2588–2612.
    1. Bernard P, Gabant P, Bahassi E M, Couturier M. Positive-selection vectors using the F plasmid ccdB killer gene. Gene. 1994;148:71–74. - PubMed
    1. Blomfield I C, Vaughn V, Rest R F, Eisenstein B I. Allelic exchange in Escherichia coli using the Bacillus subtilis sacB gene and a temperature sensitive pSC101 replicon. Mol Microbiol. 1991;5:1447–1457. - PubMed
    1. Bochner B R. Positive selection for loss of tetracycline resistance. J Bacteriol. 1980;143:926–933. - PMC - PubMed

LinkOut - more resources