Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1998 Sep;66(9):4496-8.
doi: 10.1128/IAI.66.9.4496-4498.1998.

In vivo transduction with shiga toxin 1-encoding phage

D W Acheson  1 , J Reidl, X Zhang, G T Keusch, J J Mekalanos, M K Waldor
Affiliations

In vivo transduction with shiga toxin 1-encoding phage

D W Acheson et al. Infect Immun. 1998 Sep.

Abstract

To facilitate the study of intestinal transmission of the Shiga toxin 1 (Stx1)-converting phage H-19B, Tn10d-bla mutagenesis of an Escherichia coli H-19B lysogen was undertaken. Two mutants containing insertions in the gene encoding the A subunit of Stx1 were isolated. The resultant ampicillin-resistant E. coli strains lysogenic for these phages produced infectious H-19B particles but not active toxin. These lysogens were capable of transducing an E. coli recipient strain in the murine gastrointestinal tract, thereby demonstrating that lysogens of Shiga toxin-converting phages give rise to infectious virions within the host gastrointestinal tract.

PubMed Disclaimer

Figures

FIG. 1
FIG. 1
Tn10d-bla insertions in the H-19B genome. The 3′ end of the stxAB operon is encoded on a 2.8-kb HindIII (H)-EcoRI (E) fragment (10). The orientation of the stxAB operon along with the locations of the Tn10d-bla insertions in H-19B-Ap1 and H-19B-Ap2 is shown.
See this image and copyright information in PMC

References

    1. Acheson D W K, Calderwood S B, Boyko S A, Lincicome L L, Kane A V, Donohue-Rolfe A, Keusch G T. Comparison of Shiga-like toxin I B-subunit expression and localization in Escherichia coli and Vibrio cholerae by using trc or iron-regulated promoter systems. Infect Immun. 1993;61:1098–1104. - PMC - PubMed
    1. Acheson D W K, Keusch G T. Which Shiga toxin-producing types of E. coli are important? ASM News. 1996;62:302–306.
    1. Bishai W R, Murphy J R. Bacteriophage gene products that cause human disease. In: Calendar R, editor. The bacteriophages. New York, N.Y: Plenum Press; 1988. pp. 683–724.
    1. Burghoff R L, Pallesen L, Krogfelt K A, Newman J V, Richardson M, Bliss J L, Laux D C, Cohen P S. Utilization of the mouse large intestine to select an Escherichia coli F-18 DNA sequence that enhances colonizing ability and stimulates synthesis of type 1 fimbriae. Infect Immun. 1993;61:1293–1300. - PMC - PubMed
    1. Casadaban M J. Transposition and fusion of the lac genes to select promoters in Escherichia coli using bacteriophage lambda and Mu. J Mol Biol. 1976;104:541–555. - PubMed

Publication types

LinkOut - more resources