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. 1998 Jul;19(10):1659-61.
doi: 10.1002/elps.1150191023.

Immunoblotting of multiple antigenic peptides

B T Kurien  1 , K Jackson, R H Scofield
Affiliations

Immunoblotting of multiple antigenic peptides

B T Kurien et al. Electrophoresis. 1998 Jul.

Abstract

Multiple antigenic peptides (MAPs) can be efficiently separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a nitrocellulose membrane for subsequent use in immunoblot (IgG and IgM). MAPs can be stained by Coomassie and silver on SDS-PAGE as well as by Fast Green on an immunoblot. Affinity immunoblotting for analysis of antibody clonotype distribution has also been carried out using MAPs. High performance liquid chromatography purification of the MAPs is mainly responsible for their migration as sharp bands in SDS-PAGE and immunoblotting.

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