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. 1976 Sep;8(5):501-6.
doi: 10.1007/BF01003839.

The quantification of formazans in tissue sections by microdensitometry. II. The use of BPST, a new tetrazolium salt

F P Altman

The quantification of formazans in tissue sections by microdensitometry. II. The use of BPST, a new tetrazolium salt

F P Altman. Histochem J. 1976 Sep.

Abstract

This article describes the use of a microdensitometer for the measurement of BPST formazan in tissue sections. BPST is a new tetrazolium salt, 2-(2-benzothiazolyl)-3-(4-phthalhydrazidyl)-5-styryl-tetrazolium chloride, which produces a single, well-defined formazan, and is thus easily quantified. The formazan gives an excellent localization, since BPST was originally designed for ultrastructural work. Activities are expressed in absolute units as n moles hydrogen/mm3, and are thus directly comparable with standard biochemical data.

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