doi: 10.1128/JVI.19.3.998-1005.1976.
Methylation pattern of genomic RNA from Moloney murine leukemia virus
- PMID: 972437
- PMCID: PMC354940
- DOI: 10.1128/JVI.19.3.998-1005.1976
Item in Clipboard
Methylation pattern of genomic RNA from Moloney murine leukemia virus
J Virol.
1976 Sep.
Abstract
32P- and methyl-3H-labeled 70S Moloney murine leukemia virus RNA was purified from virions produced in Moloney murine leukemia virus-infected mouse embryo cells. Primer-free RNA subunits obtained by heat treatment and zonal centrifugation were digested with RNase T2, and methylated oligonucleotides were chromatographed on DEAE-Sephadex in 7 M urea. Approximately one molecule of RNase T2-stable oligonucleotide (-5 charge) was isolated per subunit. Structural analysis indicated that the sequence of the oligonucleotide is m7GpppGmpCp. Analysis of the mononucleotide fraction isolated by DEAE-Sephadex chromatography of the RNase T2 digest identified 15 to 23 internal N6-methyladenylic acid molecules per subunit.
Similar articles
-
5'-terminus of Moloney murine leukemia virus 35s RNA is m7G5' ppp5' GmpCp.J Virol. 1976 Oct;20(1):324-9. doi: 10.1128/JVI.20.1.324-329.1976. J Virol. 1976. PMID: 185415 Free PMC article.
-
Measurement of the sequence complexity of cloned Moloney murine leukemia virus 60 to 70S RNA: evidence for a haploid genome.J Virol. 1974 Sep;14(3):421-9. doi: 10.1128/JVI.14.3.421-429.1974. J Virol. 1974. PMID: 4368895 Free PMC article.
-
Sequence homology between Moloney murine sarcoma virus and Moloney leukemia virus RNA.J Virol. 1976 Jul;19(1):82-9. doi: 10.1128/JVI.19.1.82-89.1976. J Virol. 1976. PMID: 59817 Free PMC article.
-
Low-molecular-weight RNAs of Moloney murine leukemia virus: identification of the primer for RNA-directed DNA synthesis.J Virol. 1977 Mar;21(3):1031-41. doi: 10.1128/JVI.21.3.1031-1041.1977. J Virol. 1977. PMID: 66325 Free PMC article.
-
RNA-directed DNA synthesis in Moloney murine leukemia virus: interaction between the primer tRNA and the genome RNA.J Virol. 1979 Aug;31(2):398-407. doi: 10.1128/JVI.31.2.398-407.1979. J Virol. 1979. PMID: 480476 Free PMC article.
Cited by
-
Presence of 5'-terminal cap structures in virus-specific RNA from feline leukemia virus-infected cells.J Virol. 1978 May;26(2):226-35. doi: 10.1128/JVI.26.2.226-235.1978. J Virol. 1978. PMID: 207884 Free PMC article.
-
N6-methyladenosine modification of HIV-1 RNA suppresses type-I interferon induction in differentiated monocytic cells and primary macrophages.PLoS Pathog. 2021 Mar 10;17(3):e1009421. doi: 10.1371/journal.ppat.1009421. eCollection 2021 Mar. PLoS Pathog. 2021. PMID: 33690734 Free PMC article.
-
"sarc" sequence transcription in Moloney sarcoma virus-transformed nonproducer cell lines.J Virol. 1979 Jan;29(1):76-82. doi: 10.1128/JVI.29.1.76-82.1979. J Virol. 1979. PMID: 430602 Free PMC article.
-
Regulation of antiviral innate immunity by chemical modification of viral RNA.Wiley Interdiscip Rev RNA. 2022 Nov;13(6):e1720. doi: 10.1002/wrna.1720. Epub 2022 Feb 12. Wiley Interdiscip Rev RNA. 2022. PMID: 35150188 Free PMC article. Review.
-
Analysis of cytoplasmic RNA and polyribosmomes from feline leukemia virus-infected cells.J Virol. 1978 Mar;25(3):750-63. doi: 10.1128/JVI.25.3.750-763.1978. J Virol. 1978. PMID: 205670 Free PMC article.
References
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
