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. 1976 Sep;19(3):998-1005.
doi: 10.1128/JVI.19.3.998-1005.1976.

Methylation pattern of genomic RNA from Moloney murine leukemia virus

M Bondurant, S Hashimoto, M Green

Methylation pattern of genomic RNA from Moloney murine leukemia virus

M Bondurant et al. J Virol. 1976 Sep.

Abstract

32P- and methyl-3H-labeled 70S Moloney murine leukemia virus RNA was purified from virions produced in Moloney murine leukemia virus-infected mouse embryo cells. Primer-free RNA subunits obtained by heat treatment and zonal centrifugation were digested with RNase T2, and methylated oligonucleotides were chromatographed on DEAE-Sephadex in 7 M urea. Approximately one molecule of RNase T2-stable oligonucleotide (-5 charge) was isolated per subunit. Structural analysis indicated that the sequence of the oligonucleotide is m7GpppGmpCp. Analysis of the mononucleotide fraction isolated by DEAE-Sephadex chromatography of the RNase T2 digest identified 15 to 23 internal N6-methyladenylic acid molecules per subunit.

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