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. 1998 Sep;64(9):3507-11.
doi: 10.1128/AEM.64.9.3507-3511.1998.

Pseudomonas sp. strain 273, an aerobic alpha, omega-dichloroalkaneDegrading bacterium

C Wischnak  1 , F E Löffler, J Li, J W Urbance, R Müller
Affiliations

Pseudomonas sp. strain 273, an aerobic alpha, omega-dichloroalkaneDegrading bacterium

C Wischnak et al. Appl Environ Microbiol. 1998 Sep.

Abstract

A gram-negative, aerobic bacterium was isolated from soil; this bacterium grew in 50% (vol/vol) suspensions of 1,10-dichlorodecane (1,10-DCD) as the sole source of carbon and energy. Phenotypic and small-subunit ribosomal RNA characterizations identified the organism, designated strain 273, as a member of the genus Pseudomonas. After induction with 1,10-DCD, Pseudomonas sp. strain 273 released stoichiometric amounts of chloride from C5 to C12 alpha, omega-dichloroalkanes in the presence of oxygen. No dehalogenation occurred under anaerobic conditions. The best substrates for dehalogenation and growth were C9 to C12 chloroalkanes. The isolate also grew with nonhalogenated aliphatic compounds, and decane-grown cells dechlorinated 1,10-DCD without a lag phase. In addition, cells grown on decane dechlorinated 1,10-DCD in the presence of chloramphenicol, indicating that the 1,10-DCD-dechlorinating enzyme system was also induced by decane. Other known alkane-degrading Pseudomonas species did not grow with 1,10-DCD as a carbon source. Dechlorination of 1,10-DCD was demonstrated in cell extracts of Pseudomonas sp. strain 273. Cell-free activity was strictly oxygen dependent, and NADH stimulated dechlorination, whereas EDTA had an inhibitory effect.

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Figures

FIG. 1
FIG. 1
Phylogenetic tree of Pseudomonas sp. strain 273 and its closest relatives based on 16S RNA sequence analysis.
FIG. 2
FIG. 2
Batch fermentation of strain 273 in a 10-liter bioreactor with mineral salts medium containing 5 mM 1,10-DCD as the sole carbon source. The consumption of 1,10-DCD (circles), the release of chloride (triangles), and the increase in biomass (squares) was monitored over time. pH was kept constant at 7 by automatic titration with NaOH.
FIG. 3
FIG. 3
Enzymatic conversion of 1,10-DCD (circles) and chloride release (triangles) in cell extracts of strain 273 in the presence (solid symbols) or absence (open symbols) of NADH (5 mM) and Fe2+ (0.2 mM). Data were averaged from two independent experiments.
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