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. 1998 Sep;64(9):3530-2.
doi: 10.1128/AEM.64.9.3530-3532.1998.

Influence of lipid composition on pediocin PA-1 binding to phospholipid vesicles

Y Chen  1 , R D Ludescher, T J Montville
Affiliations

Influence of lipid composition on pediocin PA-1 binding to phospholipid vesicles

Y Chen et al. Appl Environ Microbiol. 1998 Sep.

Abstract

Pediocin PA-1 bound to anionic lipid vesicles with saturated or unsaturated fatty acid chains in a lipid concentration-dependent fashion. Little change in binding parameters was observed for zwitterionic lipid vesicles. Decreasing the anionic lipid content of the vesicles gave a higher relative dissociation constant for the peptide-lipid interactions and further supports the electrostatic interaction model of binding.

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Figures

FIG. 1
FIG. 1
Influence of lipid composition on binding of pediocin PA-1 to lipid vesicles. (A) DOPG (•) and DOPC (■). (B and C) Molar ratios of DMPG to DMPC were 1:0 (•), 1:1 (▾), 1:3 (▴), and 0:1 (■). In panels A and B, the x-axis was calculated as the ratio of total lipid concentration to peptide concentration. In panel C, the x-axis was calculated by using the anionic lipid concentration instead of the total lipid concentration in curves b and c, while curves a and d were the same as curves a and d in panel B.
FIG. 2
FIG. 2
Determination of Kd/n. The datum points derived from Fig. 1A (curve a) were fitted by using the equation (ɛ − 1) = (ɛb − 1)m/(Kd/n + m). ɛ was defined as λmaxo/λmax, and m was the total lipid concentration.
See this image and copyright information in PMC

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