Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1976 Feb;3(1):135-43.
doi: 10.1111/j.1365-2125.1976.tb00580.x.

A sensitive assay of 5-fluorouracil in plasma by gas chromatography-mass spectrometry

B L Hillcoat, M Kawai, P B McCulloch, J Rosenfeld, C K Williams

A sensitive assay of 5-fluorouracil in plasma by gas chromatography-mass spectrometry

B L Hillcoat et al. Br J Clin Pharmacol. 1976 Feb.

Abstract

1 A gas chromatographic-mass spectrometric method was developed for determining 5-fluorouracil in plasma, using methylated thymine as an internal standard. 2 5-fluorouracil was extracted from plasma by a novel procedure which removed plasma components interferring with the sensitivity of the assay. The method included heating the plasma, washing with ether and extracting the drug under optimum conditions. 3 The sensitivity of the assay was 10 ng/ml plasma, sufficient to determine the low concentrations of 5-fluorouracil found in plasma during continuous infusion of the drug in patients receiving chemotherapy for cancer.

PubMed Disclaimer

References

    1. Cancer Res. 1963 Sep;23:1226-43 - PubMed
    1. Methods Biochem Anal. 1962;10:201-28 - PubMed
    1. J Chromatogr. 1974 Nov 6;99(0):519-27 - PubMed
    1. J Pharm Sci. 1969 Mar;58(3):370-1 - PubMed
    1. J Pharm Sci. 1973 Apr;62(4):572-5 - PubMed

LinkOut - more resources