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. 1998 Sep 4;434(3):272-6.
doi: 10.1016/s0014-5793(98)00994-6.

Isolation of human intestinal defensins from ileal neobladder urine

E M Porter  1 , M A Poles, J S Lee, J Naitoh, C L Bevins, T Ganz
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Free article

Isolation of human intestinal defensins from ileal neobladder urine

E M Porter et al. FEBS Lett. .
Free article

Abstract

We describe the isolation of naturally occurring human intestinal defensins HD-5 and HD-6 from ileal neobladder urine and ileal mucosa. Using an antibody-based detection assay, we found multiple N-terminally processed forms of HD-5. The predominant HD-5 forms in tissue were longer than those in neobladder urine (amino acid (aa) 23-94 and 29-94 versus aa 36-94, 56-94 and 63-94) suggesting that Paneth cells store prodefensin that is processed to mature defensin during or after degranulation. Search for mature HD-6 yielded aa 69-100 as the predominant form in both sources. The ileal neobladder is a promising model to study human Paneth cell secretion.

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