Immunohistochemical characterization of the lesions of feline progressive lymphocytic cholangitis/cholangiohepatitis

Abstract

The histopathological features of liver biopsies from 20 cats with progressive lymphocytic cholangitis/cholangiohepatitis are reported. These biopsies were subject to immunohistochemical investigation for expression of CD3, CD79. Major Histocompatibility Complex (MHC) Class II molecules, and feline IgG, IgM and IgA. Livers from five normal cats, which were also examined showed constitutive expression of MHC Class II by sinusoidal Kupffer cells and bile duct epithelium, in addition to a population of portal, and bile duct inter-epithelial, CD3+ T lymphocytes. In liver biopsies from cats with the active phase of lymphocytic cholangitis/cholangiohepatitis (n = 11), the portal lymphocytes were predominantly CD3+ T cells that infiltrated bile duct epithelium and periportal hepatic parenchyma, CD79+ B lymphocytes formed distinct aggregates or follicles within the regions of T-cell infiltration. Low numbers of plasma cells were present, and these predominantly expressed IgA. MHC Class II was expressed by Kupffer cells, infiltrating T and B lymphocytes and macrophages. There was membrane and cytoplasmic Class II expression by bile duct epithelium, some vascular endothelium, and fibroblasts within areas of fibrosis. In liver biopsies from cats with chronic lymphocytic cholangitis/cholangiohepatitis (n = 9), there was less in flammation, but the composition of the infiltrates was similar to that in the active phase of disease. The findings provide further evidence for an immune mediated pathogenesis in progressive lymphocytic cholangitis/cholangiohepatitis.