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Comparative Study
. 1976 Oct;22(10):1593-5.

Determination of serum retinol (vitamin A) by high-speed liquid chromatography

M G De Ruyter, A P De Leenheer
  • PMID: 975502
Comparative Study

Determination of serum retinol (vitamin A) by high-speed liquid chromatography

M G De Ruyter et al. Clin Chem. 1976 Oct.

Abstract

We report a fast and simple high-speed liquid-chromatographic assay for serum retinol. Only 100 mul of serum is required. The lower detection limit is 50 mug/liter; linearity was demonstrated up to 1.50 mg/liter. On analyzing a serum pool eight times, a CV of 2.5% was obtained. Values by this method are compared with results obtained by a flurometric method [Clin. Chem. 16, 766(1970)].

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