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. 1998 Oct;64(10):3663-8.
doi: 10.1128/AEM.64.10.3663-3668.1998.

A plant growth-promoting bacterium that decreases nickel toxicity in seedlings

GI Burd  1 , DG Dixon, BR Glick
Affiliations

A plant growth-promoting bacterium that decreases nickel toxicity in seedlings

GI Burd et al. Appl Environ Microbiol. 1998 Oct.

Abstract

A plant growth-promoting bacterium, Kluyvera ascorbata SUD165, that contained high levels of heavy metals was isolated from soil collected near Sudbury, Ontario, Canada. The bacterium was resistant to the toxic effects of Ni2+, Pb2+, Zn2+, and CrO4-, produced a siderophore(s), and displayed 1-aminocyclopropane-1-carboxylic acid deaminase activity. Canola seeds inoculated with this bacterium and then grown under gnotobiotic conditions in the presence of high concentrations of nickel chloride were partially protected against nickel toxicity. In addition, protection by the bacterium against nickel toxicity was evident in pot experiments with canola and tomato seeds. The presence of K. ascorbata SUD165 had no measurable influence on the amount of nickel accumulated per milligram (dry weight) of either roots or shoots of canola plants. Therefore, the bacterial plant growth-promoting effect in the presence of nickel was probably not attributable to the reduction of nickel uptake by seedlings. Rather, it may reflect the ability of the bacterium to lower the level of stress ethylene induced by the nickel.

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Figures

FIG. 1
FIG. 1
Influence of Ni2+ on the growth of K. ascorbata SUD165. Cells were grown for 18 h at 25°C in TLP medium plus gluconate.
FIG. 2
FIG. 2
Influence of Ni2+ on canola seedling development in growth pouches. The error bars represent 1 SEM. Values differ significantly (P < 0.001) from those for the control group when [Ni2+] is 1 mM or greater. Data were analyzed by ANOVA with 50 to 60 seedlings in each treatment group. The results of a typical experiment are shown.
FIG. 3
FIG. 3
Influence of Ni2+ on canola seedling development in pots. The error bars represent 1 SEM. Values differ significantly (P < 0.001) from those for the control group when [Ni2+] is 5 mM or greater. Data were analyzed by ANOVA with 55 to 60 seedlings in each treatment group. The results of a typical experiment are shown.
FIG. 4
FIG. 4
Influence of Ni2+ on tomato seedling development in pots. The error bars represent 1 SEM. Values differ significantly (P < 0.001) from those for the control group at all Ni2+ concentrations. Data were analyzed by ANOVA with 55 to 60 seedlings in each treatment group. The results of a typical experiment are shown.
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