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. 1998 Oct;64(10):3972-6.
doi: 10.1128/AEM.64.10.3972-3976.1998.

Enzymatic conversion of glucose to UDP-4-keto-6-deoxyglucose in Streptomyces spp

S Y Liu  1 , J P Rosazza
Affiliations

Enzymatic conversion of glucose to UDP-4-keto-6-deoxyglucose in Streptomyces spp

S Y Liu et al. Appl Environ Microbiol. 1998 Oct.

Abstract

All of the 2,6-dideoxy sugars contained within the structure of chromomycin A3 are derived from D-glucose. Enzyme assays were used to confirm the presence of hexokinase, phosphoglucomutase, UDPG pyrophosphorylase (UDPGP), and UDPG oxidoreductase (UDPGO), all of which are involved in the pathway of glucose activation and conversion into 2,6-dideoxyhexoses during chromomycin biosynthesis. Levels of the four enzymes in Streptomyces spp. cell extracts were correlated with the production of chromomycins. The pathway of sugar activation in Streptomyces spp. involves glucose 6-phosphorylation by hexokinase, isomerization to G-1-P catalyzed by phosphoglucomutase, synthesis of UDPG catalyzed by UDPGP, and formation of UDP-4-keto-6-deoxyglucose by UDPGO.

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Figures

FIG. 1
FIG. 1
Structures of chromomycins A2 and A3.
FIG. 2
FIG. 2
Proposed pathway for the formation of 2,6-dideoxy sugars in streptomycetes involving HK, PGM, UDPGP, and UDPGO.
FIG. 3
FIG. 3
Relationships of HK (■), PGM (▵), UDPGP (×10) (□), and UDPGO (×10) (▴) to production of chromomycin A3 (•) by Streptomyces sp. strain MRS202. The results are the averages of duplicate assays within a deviation of no more than 4%.
See this image and copyright information in PMC

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